avirulent strain meaning in English
减毒株，无毒株
无毒毒株
无毒菌株
无毒株
Examples
- Now many laboratories have used avirulent strains of s . typhimurium to deliver h . pylori antigens as a way of stimulating an immune response to those determinants
以鼠伤寒沙门氏菌标准株基因组dna作为模板，采用pcr的方法克隆了鼠伤寒沙门氏菌asd基因，经序列分析与文献报道一致。 - Newcastle disease virus ( ndv ) strain 695 , a thermostable nature avirulent strain , were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene , a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用从国外引进的新城疫热稳定性天然弱毒b _ ( 95 )株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna ，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应( rt - pcr )扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t - a克隆技术将其克隆到pgem - t - easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。 - From dead chickens , one virrus was isolated by using eggs and chicken embryo fibroblast . lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum . this strain ' s biological property was tested by barren spot , cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本实验采用spf鸡胚及鸡胚原代成纤维细胞，从河北省某鸡场新城疫免疫抗体很高的病死鸡的脑组织中分离得到一株病毒。此株病毒能凝集鸡的红细胞，并且这种凝集可以被特异性抗血清所抑制。